PUNICALAGIN AND PUNICALIN SUPPRESS THE ADIPOCYTE DIFFERENTIATION THROUGH THE TRANSCRIPTION FACTORS
M. Berkoz1,*, S. Yalin2, M. Yildirim3, A.E. Yalın2, Ü. Çömelekoğlu4
1Yuzuncu Yil University – Department of Biochemistry, Van, 2 Mersin University, Faculty of Pharmacy -Department of Biochemistry, Mersin,
3 Tarsus University, Healthcare Vocational School – Pharmacy Services
Program, Tarsus, 4 Mersin University, Faculty of Medicine – Department of Biophysics, Mersin, Turkey
Background—Pomegranate is a rich source of many polyphenolic compounds including ellagitannins (punicalagin, punicalin and others).
Aim. The effects of punicalagin and punicalin on adipogenesis were investigated in this study.
Materials and Methods. To examine the effect of punicalagin and punicalin on adipocyte differentiation, various concentrations of punicalagin and punicalin (2-10 µM) were applied to differentiated 3T3-L1 cells. Glyceraldehyde-3-phosphate dehydrogenase (GPDH) activity, Oil red O staining, intracellular triglyceride levels, and gene expressions of transcription factors (Peroxisome proliferator-activated receptor-γ (PPARγ), CCAAT enhancer-binding proteins-α (C/EBPα), Sterol regulatory element-binding protein 1c (SREBP-1c)) and lipolysis associated genes (hormone-sensitive lipase (HSL), Perilipin A, tumor necrosis factor-α (TNF-α)) were examined in order to investigate the effects of punicalagin and punicalin on adipocyte differentiation. Results. Punicalagin and punicalin applications caused a continuous decrease in cell size and intracellular triglyceride accumulation. GPDH activity and transcription gene expressions decreased significantly in groups that were applicated punicalagin and punicalin at high concentrations. Punicalagin, but not punicalin, down-regulated the expression of HSL and perilipin A and up-regulated the expression of TNF-α in a dose-dependent manner. In conclusion, both punicalagin and punicalin were able to inhibit the adipocyte differentiation.
Keywords: 3T3-L1, adipogenesis, punicalagin, punicalin, transcription factors, cell differentiation.